Why zebra fish is used for research?
As zebrafish eggs are fertilised and develop outside the mother’s body it is an ideal model organism for studying early development. Zebrafish have a similar genetic structure to humans. They share 70 per cent of genes with us. 84 per cent of genes known to be associated with human disease have a zebrafish counterpart.
What is synteny analysis?
Synteny analysis is one of the most important fields in comparative genome analysis as it is the basis of evolutionary studies at both the gene and genome levels. Most practically, it helps improve the gene annotation of newly sequenced genomes.
What are 3 reasons that zebra fish make good model organisms?
Because of its fully sequenced genome, easy genetic manipulation, high fecundity, external fertilization and rapid development, and nearly transparent embryo, zebrafish are a unique model animal for biomedical research, including studies of biological processes and human diseases.
How zebra fish is genetically modified?
The most commonly used method in zebrafish involves the microinjection of embryos with a plasmid DNA (construct) that is comprised of the transposase mRNA and transposon (often Tol2). The insertion can then be passed on to approximately 50% of the progeny.
How much DNA do we share with zebrafish?
Humans and zebrafish share 70 percent of the same genes and 84 percent of human genes known to be associated with human disease have a counterpart in zebrafish.
Is zebrafish transparent?
Zebrafish embryos are transparent, permitting an unprecedented level of direct observation during embryonic development. Relating to our research interests, this transparency allows us to easily observe circulatory function in live embryos (bottom left).
What is synteny mapping?
Synteny maps. (A) For each Tetraodon chromosome, colored segments represent conserved synteny with a particular human chromosome. Synteny is defined as groups of two or more Tetraodon genes that possess an orthologue on the same human chromosome, irrespective of orientation or order.
What do you mean by synteny?
A term used to describe the state of two or more genes being present on the same chromosome, though not necessarily linked.
Why zebrafish is considering as an emerging model?
Zebrafish provides a series of advantages as a model of study due to its rapid development, optical transparency, high number of offspring and straightforward strategies for forward and reverse genetic manipulation.
Does CMV promoter work in zebrafish?
Common promoters such as the CMV promoter work well in zebrafish and allow the expression of the protein of interest. To achieve cell type-specific expression, many well-characterized enhancer elements for zebrafish are available, which can be combined, for example with an ORF encoding a fluorescent protein.
How much DNA do we share with chimps?
These three species look alike in many ways, both in body and behavior. But for a clear understanding of how closely they are related, scientists compare their DNA, an essential molecule that’s the instruction manual for building each species. Humans and chimps share a surprising 98.8 percent of their DNA.
Does the zebrafish genome have a syntenic relationship to the human genome?
This work now provides a syntenic relationship to the human genome for the majority of the zebrafish genome. Publication types Comparative Study Research Support, Non-U.S. Gov’t
Are zebrafish larvae sensitive to stimuli?
It is well known that zebrafish larvae are sensitive to a variety of stimulus modalities, including touch, olfaction, chemosensation, audition, vestibular inputs, heat, and vision [ 24 ]. Among the behaviors, locomotion or swimming in zebrafish larvae is increasingly gaining attention in neuropharmacology.
What is conserved synteny analysis?
Conserved synteny analysis provides a method that helps overcome such problems. Here we describe conserved synteny analysis for zebrafish genes and discuss the Synteny Database, a website specifically designed to identify conserved syntenies for zebrafish that takes into account the teleost genome duplication (TGD).
Do convulsant drugs affect locomotor activity in zebrafish larvae?
Similarly, convulsant drugs such as 4-aminopyridine, a potassium channel blocker and aconitine, a voltage gated sodium channel agonist, also induced increased locomotor activity in zebrafish larvae in the dark condition [ 90 ]. Thus, the convulsant drugs showed similar effects in zebrafish larvae.