Is Bacillus megaterium endospore positive or negative?
Bacillus megaterium is a rod-like, Gram-positive, mainly aerobic spore forming bacterium found in widely diverse habitats. With a cell length of up to 4 µm and a diameter of 1.5 µm, B.
What color does Bacillus megaterium stain?
Answer and Explanation: While staining Bacillus megaterium for cell wall staining, the Gram-positive bacteria are observed purple and Gram-negative bacteria are observed pink… See full answer below.
Is Bacillus megaterium Gram stain?
megaterium is a Gram-positive, aerobic spore-forming neutralophilic bacterium found in diverse habitats but commonly regarded as a soil bacterium. Its ability to utilize different carbon sources and grow at a wide temperature range (3 °C to 45 °C) makes it an ideal industrial organism9,10.
What would you observe if you performed an endospore stain on a culture of Bacillus megaterium but neglected to apply any heat during the staining process?
What would you observe if you performed an endospore stain on a culture of bacillus megaterium but neglected to apply any heat during the staining process? only pink safranin staining. cowboys, ranch hands, shepherds, and persons who work tanning leather are all at an increased risk for being infected with anthrax.
Does Bacillus megaterium produce endospores?
Bacillus megaterium is a Gram-positive, rod shaped Endospore-forming Bacteria. It is considered Aerobic, but, it is also capable of growing under anaerobic conditions when necessary.
Is Bacillus megaterium shape?
Bacillus megaterium is a gram positive, spore producing bacteria. It is a Eubacteria and is found in the soil. It has a rod shape and is one of the largest Eubacteria. Colonies form in chains due to sticky polysaccharides on the cell wall.
How do you identify a Bacillus megaterium?
megaterium are concave, smooth and milk white, while B. mycoides looks white, coarser with fine threads around the colony and the color is milk white. The cell morphology (Figure 3 and Table 1) shows that the cell is rod-shaped, gram positive and sporous.
What is the shape of Bacillus megaterium?
Bacillus megaterium is a gram positive, spore producing bacteria. It is a Eubacteria and is found in the soil. It has a rod shape and is one of the largest Eubacteria.
Is Bacillus megaterium a characteristic?
What does a positive result for the endospore stain indicate about the organism?
a. What does a positive result for the endospore stain indicate about the organism? -If you get a positive result for the endospore stain, it indicates (barring contamination) that the organism produces spores.
Does B. megaterium produce spores?
B. megaterium comprises a number of morphologically distinct strains sharing the unusual large dimensions of both cells (length up to 4 μm and a diameter of 1.5 μm) and spores (length up to 3 μm and diameter of 1 μm) (Di Luccia et al., 2016).
How to stain Bacillus megaterium endospores under 1000x objective?
Bacillus megaterium endospores under 1000x objective. The endospore structure of the Bacillus megaterium was examined by employing the Schaeffer-Fulton Spore staining method. The method applied a differential staining method by which the spores of the bacterial culture was differentiated from other structure.
What is endospore staining?
Endospore staining is a differential staining technique that selectively stains the spores and makes them distinguishable from the vegetative part of the cells. Endospores are produced by a few genera of Gram-positive bacilli such as Bacillus and Clostridium, in response to adverse environmental conditions.
How do you identify endospores and vegetative cells?
At the end of the staining process, vegetative cells will be pink, and endospores will be dark green. Spores may be located in the middle of the cell, at the end of the cell, or between the end and middle of the cell.
What is the principle of spore stain?
Principle of Spore Staining: A primary stain (malachite green) is used to stain the endospores. Because endospores resist staining, the malachite green will be forced into (i.e, malachite green permeate the spore wall) the endospores by heating. In this technique heating acts as a mordant.