What is the A549 cell line?
A549 cells are lung carcinoma epithelial cells that constitute a cell line. A549 is one of the commonly used cell lines for a wide range of research applications.
What type of cancer is A549?
One commonly used line is A549, an epithelial carcinoma derived from a 58 year old male patient, known to be KRAS mutant and EGFR wild type. Lung cancer is the leading cause of cancer-related deaths in the world with non-small cell lung cancer (NSCLC) making up about 85% of all lung cancer cases.
What is ATCC in cell culture?
ATCC or the American Type Culture Collection is a nonprofit organization which collects, stores, and distributes standard reference microorganisms, cell lines and other materials for research and development.
How long does it take for A549 cells to adhere?
A549 cells doubling time is approximately 22 hours. Split confluent A549 cell culture 1:4 to 1:9 every 4-7 days with trypsin/EDTA and renew complete medium every 2-3 days.
Where are A549 cells found?
lung tissue
A549 cells, as found in the lung tissue of their origin, are squamous and responsible for the diffusion of some substances, such as water and electrolytes, across alveoli. If A549 cells are cultured in vitro, they grow as a monolayer; adherent or attaching to the culture flask.
What is the purpose of ATCC?
The American Type Culture Collection (ATCC) is a private, nonprofit organization dedicated to the acquisition, preservation, authentication, and distribution—the “APAD” activities—of diverse biological materials.
What is ATCC What does it do?
ATCC (American Type Culture Collection) is a private, nonprofit, global biological resource center and standards organization that provides scientists with the biomaterials and resources they need to conduct critical life science research.
How long does it take for fibroblasts to attach?
BioVision Inc. I agree, it depends on the cells type. I have cultured neural stem cell line, and lung and skin fibroblasts and they attach in 2-3 hours. But My MCF-7 cell line is not attaching even after 4hours.
How long can cells survive in PBS?
All Answers (4) Do not let your cells rest in PBS for more than 20-25 mins. They will lose their adhesion molecules and half of them wont attach to plastic especially the MSCs. usually cell lines are not kept in PBS for longer time not more than 5mins to maintain them in healthy state.
Are HepG2 cells hepatocytes?
However, their major limitation is the lower expression of some metabolic activities compared with hepatocytes. HepG2 is a human hepatoma that is most commonly used in drug metabolism and hepatotoxicity studies.