What is hygromycin used for?
Hygromycin B has been used to select transfectants in a wide variety of cells including bacteria, protozoans, yeast, fungi, plants, and mammalian cells. Resistance to Hygromycin B is conferred by a gene coding for a phosphotransferase that phosphorylates Hygromycin B, thereby inactivating it.
What is hygromycin resistance gene?
The resistance gene is a kinase that inactivates hygromycin B through phosphorylation. Since the discovery of hygromycin-resistance genes, hygromycin B has become a standard selection antibiotic in gene transfer experiments in many prokaryotic and eukaryotic cells.
Is hygromycin an antibiotic?
Hygromycin B is an aminoglycoside antibiotic produced by Streptomyces hygroscopicus (Mann and Bromer 1958).
What concentration of hygromycin for selection?
100-1000 µg/ml
The concentration for selection ranges from 100-1000 µg/ml (typically 200 µg/ml) and should be optimized for each cell line.
How long does hygromycin selection take?
Cells transfected with a plasmid harboring the hygromycin resistance (hph) gene should be grown in complete growth medium for 48-72 hours post-transfection before selection antibiotic is applied.
How long is hygromycin stable?
What is the stability of Hygromycin B in solution? The antibiotic is stable for at least 2 years at 4°C. It is stable for about one month at 37°C.
How do you dissolve hygromycin B?
Hygromycin B is soluble in water at >50 mg/mL. 2 It is also soluble in methanol or ethanol. Solutions should be sterilized by filtration, not by autoclaving. Hygromycin B is dissolved in water at 50 mg/mL, then sterile-filtered to produce H 0654, or γ-irradiated to produce H 5527.
How long do hygromycin plates last?
The antibiotic is stable for at least 2 years at 4°C. It is stable for about one month at 37°C.
Is hygromycin B light sensitive?
The molecules of hygromycin are still light sensitive, no matter how much is there… you need to protect it from light.
Does hygromycin expire?
How long does it take to select hygromycin?
Is hygromycin light sensitive?