What Colour is Cy3?
The Cy3 dye is a traditional orange-fluorescent label for protein and nucleic acid conjugates used for imaging, flow cytometry, and genomic applications. It is also the basis for the classic lipophilic tracer DiI and its variants.
What color is Alexa Fluor 594?
Invitrogen Alexa Fluor 594 dye is a bright, red-fluorescent dye that can be excited using the 561 nm or 594 nm laser lines.
Is Cy3 a fluorophore?
Cy®3 is an orange fluorophore commonly used in applications such as immunolabeling, nucleic acid labeling, fluorescence microscopy, and flow cytometry. The dye has an absorption wavelength that peaks around 548-552 nm, and an emission maximum around 562-570 nm.
What color does Cy3 fluorescence?
greenish yellow
Cy3 fluoresces greenish yellow (~550 nm excitation, ~570 nm emission), while Cy5 is fluorescent in the far-red region (~650 excitation, 670 nm emission). Cy3 can be detected by various fluorometers, imagers, and microscopes with standard filters for Tetramethylrhodamine (TRITC).
Is Cy5 far-red?
Invitrogen Cy5 dye is a bright, far-red-fluorescent dye with excitation ideally suited for the 633 nm or 647 nm laser lines.
What color is Alexa Fluor 633?
-red dye
Alexa Fluor™ 633 is a bright and photostable far-red dye with excitation ideally suited to the 633 nm laser line.
What does Texas red stain?
Texas Red or sulforhodamine 101 acid chloride is a red fluorescent dye, used in histology for staining cell specimens, for sorting cells with fluorescent-activated cell sorting machines, in fluorescence microscopy applications, and in immunohistochemistry.
Is FAM same as FITC?
FITC and FAM are both derivatives of fluorescein. FITC is fluorescein with an isothiocyanate reactive group added, while 6-FAM is 6-carboxyfluorescein. FITC and 6-FAM have identical spectral characteristics. In experiments, 6-FAM performs exactly like FITC.
What does Cy3 stain for?
Can you use Cy3 and Cy5 together?
Cy3 dyes are yellow-orange. Specific filter wavelengths (550-600 nm and 655-695 nm) have to be used to avoid background contamination when using Cy3 and Cy5 together to optically separate the two fluorescent cyanines. Cy3 is used interchangeably with PE, though PE remains a better choice.
What is the Cy3 fluorophore used for?
The Cy3 fluorophore is also the basis for the lipophilic neuronal and long-term DiI cell tracing reagents, which append alkyl tails (≥12 carbons) onto the core fluorophore.
What is the nm range of the fluorophore?
FluorophoreReference Guide 400 nm 425 nm 450 nm 475 nm 500 nm 525 nm 550 nm 575 nm 600 nm 625 nm 650 nm Laser Lines ArI 488 nm ArI 514 nm DPSSL (µGreen) 532 nm GHeNe 543 nm YHeNe 594 nm Red diode 635 nm Broad UV Excitation 290-365 nm Emission Filters
How many sets of chlorophyll are in Cy3?
Click to view sets ChlorophyllA 480 650 Click to view sets Cy2™ 492 507 Click to view sets Cy3.5™ 578 591 Click to view sets Cy3™ 554 566 Click to view sets Cy5.5™ 672 690 Click to view sets Cy5™ 649 666 Click to view sets Cy7™ 753 775 Click to view sets CyQUANT GR-DNA 502 523 Click to view sets DAF-FM-NO 495 519 Click to view sets DAPI
What is Cy3 dye used for?
Cy3 dye. Invitrogen Cy3 dye is a bright, orange-fluorescent dye that can be excited using the 532 nm laser line and visualized with TRITC (tetramethylrhodamine) filter sets. In addition to immunocytochemistry applications, Cy3 dye is commonly used to label nucleic acids. The Cy3 fluorophore is also the basis for the lipophilic neuronal