What is MBP antibody?
Anti-MBP Monoclonal Antibody is a murine anti-maltose binding protein (MBP) antibody, isotype IgG2a. High purity and specificity for MBP tag. Potent: Recommended dilution is 1:10,000. Verified for use in both Western blotting and ELISA.
What is the significance of MBP in the CSF?
This test provides a measure of myelin fragments released into the spinal fluid as a result of the breakdown of myelin during acute phases in the course of demyelinating disease of the CNS (most common example of which is multiple sclerosis).
What is MBP in multiple sclerosis?
Myelin basic protein (MBP) is an abundant protein in central nervous system (CNS) myelin. MBP has long been studied as a factor in the pathogenesis of the autoimmune neurodegenerative disease multiple sclerosis (MS). MS is characterized by CNS inflammation, demyelination, and axonal loss.
What is MBP a marker for?
Myelin basic protein (MBP) is a major myelin constituent produced by oligodendrocytes in the central nervous system (CNS). Expression of MBP was considered to be a marker for oligodendrocyte differentiation and myelination in the developing CNS.
What is MBP biology?
Maltose-binding protein (MBP) is a part of the maltose/maltodextrin system of Escherichia coli, which is responsible for the uptake and efficient catabolism of maltodextrins. It is a complex regulatory and transport system involving many proteins and protein complexes.
What causes high myelin basic protein?
Multiple sclerosis is the most common cause for this, but other causes may include: Bleeding of the central nervous system. Central nervous system trauma. Certain brain diseases (encephalopathies)
What does low myelin basic protein mean?
Myelin basic protein levels between 4 to 8 ng/mL may be a sign of a chronic breakdown of myelin. It may also indicate recovery from an acute episode of myelin breakdown.
What is a normal IgG synthesis rate?
IgG synthesis rates were calculated by the isotope exchange method and compared to values obtained with the empirical formula. There was excellent concordance, from a low rate of synthesis of 5 mg per day to a high rate of 120 mg per day.
Is MBP a membrane protein?
Among these, MBP is probably the most frequently used fusion protein for membrane proteins.
What is a normal myelin basic protein?
Normal Results In general, there should be less than 4 ng/mL of myelin basic protein in the CSF.
Do neurons express MBP?
Golli-MBP is highly expressed prenatally in neurons and oligodendrocytes, even before the process of myelination begins (Tosic et al., 2002) and has been called a “molecular link” between the nervous and immune systems (Pribyl et al., 1993).
What is an MBP fusion protein?
Abstract. Maltose-binding protein (MBP) is one of the most popular fusion partners being used for producing recombinant proteins in bacterial cells. MBP allows one to use a simple capture affinity step on amylose-agarose columns, resulting in a protein that is often 70-90% pure.
What are Millipore’s MBP antibodies?
Millipore’s MBP antibodies demonstrate specificity against Myelin basic protein (MBP). See below for related products for MBP, based on the expertise of Upstate & Chemicon. Millipore’s MBP antibodies demonstrate specificity against Myelin basic protein (MBP).
Can an antibody enzyme contribute to the site-specific degradation of neural antigen?
This report describes “an antibody enzyme” (abzyme) contribution to the site-specific degradation of a neural antigen. We detected proteolytic activity toward myelin basic protein (MBP) in the fraction of antibodies purified from the sera of humans with multiple sclerosis (MS) and mice with induced experimental allergic encephalomyelitis.
What is the effect of methylated MBP on the myelin membrane?
Note=The reduction in the surface charge of citrullinated and/or methylated MBP could result in a weakened attachment to the myelin membrane. This mechanism could be operative in demyelinating diseases such as chronical multiple sclerosis (MS), and fulminating MS (Marburg disease).
Do human and murine autoantibodies cleave MBP?
Both human and murine autoantibodies appeared specifically to cleave MBP (Fig. 2A), but they did not exert any detectable proteolytic activity toward recombinant extracellular domain of MOG (Fig. 2B). Open in a separate window Fig. 2. MBP degradation by purified autoantibodies.