How does the Phadebas test work?
The test used to identify amylase uses Phadebas®, consisting of starch microspheres with a blue dye cross-linked to the starch. In the presence of amylase the starch is digested, releasing the water soluble dye into solution. The resulting blue colour is read semi-quantitatively using a spectrophotometer.
How do you test salivary amylase?
An amylase urine test may be ordered along with or after an amylase blood test. Urine amylase results can help diagnose pancreatic and salivary gland disorders. One or both types of tests may be used to help monitor amylase levels in people who are being treated for pancreatic or other disorders.
What is the color change expected for a positive Phadebas test?
For our experiments, the blood contaminated samples were diluted to approximately 1.5 ml. Added 8 µL of sample extract and read the result at 10 minutes. A yellow color change was a positive result while no color change indicated a negative result.
What is Phadebas reagent?
Phadebas is a synthetic biochemical substrate used for both qualitative and quantitative assessment of the α-amylase enzyme. Its active component is DSM-P, microspheres in which a blue dye has been chemically bound.
Is Phadebas test confirmatory?
Phadebas, therefore, cannot confirm that saliva is present upon the surface of interest, it simply suggests that there is amylase activity present.
Does amylase blood test require fasting?
It is not usually necessary to fast or make any specific preparations before having an amylase blood test. However, some medications can increase amylase levels, which can make the test results difficult to interpret.
What are the symptoms of high amylase?
These include excessive thirst, frequent urination, extreme tiredness (fatigue), and weight loss. This is often temporary. Symptoms of pancreatitis may include nausea, sweating and weakness. You may also notice pain in the middle of your chest, which may move or radiate to your back.
What is a normal amylase level?
The normal range is 40 to 140 units per liter (U/L) or 0.38 to 1.42 microkat/L (µkat/L). Normal value ranges may vary slightly among different laboratories.
What happens if amylase is high?
High amylase levels are typically a sign of acute or chronic pancreatitis. Acute pancreatitis can cause amylase levels to become four to six times higher than the upper limit of the normal range. Other conditions may cause amylase levels to increase, including: pancreatic cancer.