What is comparative genomic hybridization PPT?
Comparative genomic hybridization is a molecular cytogenetic method for analysing copy number variations (CNVs) relative to ploidy level in the DNA of a test sample compared to a reference sample, without the need for culturing cells.
What is array based comparative genomic hybridization?
Array-based comparative genomic hybridization (array CGH), also called microarray analysis, is a new cytogenetic technology that evaluates areas of the human genome for gains or losses of chromosome segments at a higher resolution than traditional karyotyping.
What is microarray comparative genomic hybridization?
Array comparative genomic hybridization (also microarray-based comparative genomic hybridization, matrix CGH, array CGH, aCGH) is a molecular cytogenetic technique for the detection of chromosomal copy number changes on a genome wide and high-resolution scale.
What is comparative genomic hybridization used for?
Comparative genomic hybridization (CGH) is a method that can be used on DNA extracted from routinely fixed tissue to assess the entire genome for the presence of changes in DNA copy number. CGH analysis has revealed that melanoma differs from melanocytic nevi by the presence of frequent chromosomal aberrations.
What is genomic array?
Genome tiling arrays consist of overlapping probes designed to densely represent a genomic region of interest, sometimes as large as an entire human chromosome. The purpose is to empirically detect expression of transcripts or alternatively spliced forms which may not have been previously known or predicted.
What can array CGH detect?
Array CGH (also known as microarray, or chromosome microarray (CMA)) is an ultra-high resolution way of objectively and quantitatively detecting whether a patient’s DNA has losses (deletions) or gains (duplications, triplications etc) which are pathogenic and therefore explain their clinical problems.
How does Array CGH work?
Array CGH compares your child’s DNA with a control DNA sample and identifies differences between the two sets of DNA. In this way, deletions or duplications (imbalances) in your child’s DNA can be identified. From this, the gene content of any such imbalance can be established.
What is microarray hybridization?
The process in which the cDNA molecules bind to the DNA probes on the slide is called hybridization. Following hybridization, the microarray is scanned to measure the expression of each gene printed on the slide.
What is an array CGH test?
Array CGH is sometimes called a microarray test. It is a detailed chromosome test that looks to see if your baby has missing genetic material (deletion) or extra genetic material (duplication). Having too much or too little genetic material can sometimes cause problems with physical and/or intellectual development.
What is array-comparative genomic hybridization?
Array-comparative genomic hybridization (also CMA, Chromosomal microarray analysis, microarray-based comparative genomic hybridization, array CGH, a-CGH, aCGH) is a technique to detect genomic copy number variations at a higher resolution level than chromosome- based comparative genomic hybridization (CGH).
What is comparative genomic hybridization (CGH)?
Comparative genomic hybridization is a molecular cytogenetic method for analysing copy number variations (CNVs) relative to ploidy level in the DNA of a test sample compared to a reference sample, without the need for culturing cells CGH in the PGD program: u000ba new tool for improved IVF outcomes?
What is array CGH and how does it work?
Array CGH applications are mainly directed at detecting genomic abnormalities in cancer. However, array CGH is also suitable for the analysis of DNA copy number aberrations that cause human genetic disorder. That is, array CGH is employed to uncover deletions, amplifications, breakpoints and ploidy abnormalities.
What is the aim of a comparative DNA analysis?
• The aim is to quickly and efficiently compare two genomic DNA samples arising from two sources which are mostly often closely related.