Why is blue dextran used in chromatography?
Bearing Cibacron Blue F3G-A, blue dextran is used in affinity chromatography and protein chromatography allowing for the identification of proteins (purified or in crude cellular extracts), and improving the purification procedures for various proteins (1), as well as in gel chromatography as molecular weight markers …
What Colour is blue dextran?
Figure 1 shows visual chromatographic separation of different components of the mixture (green in colour) containing blue dextran (blue in colour), -chymotrypsinogen (colourless) and potassium ferricyanide (yellow in colour) on a Sephadex G-75 column.
What is blue dextran?
Blue Dextran is a high-molecular-weight glucose polymer (original mol wt 2 x 10(6) g/mol) containing covalently bonded Reactive Blue 2 dye (approximately mmol/g dextran). This blue dye is known for its high binding affinity to a wide variety of proteins, with a particularly high affinity for serum albumin.
What is the significance of using blue dextran in molecular exclusion chromatography?
Blue dextran is also used for checking the column packing. A symmetrical peak of elution indicates homogeneity of packing. The inner volume (Vi) of the column can be determined by subtracting the void volume from the elution volume of small molecules such as glucose or tyrosine having K d — 1.0.
What is the elution volume of blue dextran?
Table 2
| Test molecule | Mass (Daltons) | Elution volume (ml) |
|---|---|---|
| Mineralized dentin powder | ||
| Blue Dextran | 2000000 | 10.8 (0.10)a |
| BSA | 67000 | 11.0 (0.06)a,b |
| Ovalbumin | 45000 | 10.9 (0.06)a,b |
What is the size of blue dextran?
The Vo of a given column is based on the volume of effluent required for the elution of a large molecule such as blue dextran (molecular mass of ∼2,000 kDa, Catalog Number D4772).
What is the function of dextran?
Dextran is a medication used in managing and treating various clinical conditions, including during hemorrhage, shock, surgical procedures, radiological imaging, antithrombotic administration, and ophthalmic relief of xerophthalmia.
What is the void volume of blue dextran?
Void Volume (Vo) Determination – Dissolve the blue dextran in equilibration buffer containing 5% glycerol at a concentration of 2 mg/ml. This concentration of blue dextran will give an A280 of ∼1.0 in the peak fraction.
What is Vo VE and VT?
Vo = Elution volume of a large “totally excluded” molecule such as blue dextran. Vt = Physical volume of column. Calculation of Ve. For a molecule that can partially enter the pores: Ve = Vo + Kd (Vs)
Is Blue dextran a polyelectrolyte?
Blue dextrans are polyelectrolytes due to the ionic dye ligands, and this strongly influences their properties in aqueous solution. The intrinsic viscosity decreases with increasing solvent ionic strength. Reduced viscosity g red as a function of concentration c for blue dextran 2000 in solutions of increasing ionic strength. …
What are blue-dextrans?
Blue-dextrans are blue, odourless powders which are freely soluble in water or electrolyte solutions. The product has a pronounced amphoteric character by virtue of the presence of both sulphonate and amino groups in Cibacron Blue. Since this property may influence the hydrodynamic volume in
What are the reaction conditions that affect Blue dextran properties?
Effect of reaction conditions on blue dextran properties and the hydroxyl group of the solvent water. Not unexpectedly, ( Table 1 ). Higher base concentrations appear to significantly ( Table 1 ). 3.2. Viscosity behavior ( Fig. 1 ). As polymer concentrations decrease from high increases with further decreasing concentration. At very low poly-
What is Aldrich Blue dextran 2000?
Commercially available blue dextran 2000 (Aldrich). increasing dissociation of the ionic groups (dye ligands). Increasing at low polymer concentration. At concentrations below this maxi-